Fluorescence Studies of Coenzyme-binding to Beef Heart Lactic Dehydrogenase
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چکیده
منابع مشابه
Fluorescence studies of coenzyme-binding to beef heart lactic dehydrogenase.
Among the many methods which are available for studying the binding of substances to proteins (l), those applied most commonly for pyridine nucleotide-binding to dehydrogenases have been the spectrophotometric (2) and ultracentrifuge measurements (3). An additional method has been introduced through the observations of Boyer and Theorell (4) who found that reduced diphosphopyridine nucleotide i...
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Previously reported results of ultracentrifugal separation experiments (1) have indicated that approximately 4 moles of diphosphopyridine nucleotide are bound by 1 mole of crystalline beef heart lactic dehydrogenase. A similar result was obtained by Pfleiderer et al. (2) and by van Eys et al. (3) from measurements of the absorbancy changes accompanying the formation of various adducts with enzy...
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THE reduction of methylene blue by lactic acid, or in other words, the dehydrogenation of lactic acid in the presence of extracts from animal tissues, is now known to be catalysed by a system composed of two enzymes (lactic dehydrogenase and diaphorase flavoprotein) and cozymase [Dewan & Green, 1938; Euler & Hellstrom, 1938]. The chemical reactions involved in this process may be written [Corra...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1959
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)70049-5